Foam fractionation is often considered an ineffective way of removing organic matter from freshwater due to the low surface tension of the water. In this project ozone was applied together with skimming technology. The removal efficiencies were within the same range as those observed in previous studies conducted with foam fractionators in saltwater systems (with or without ozone), corroborating that foam fractionation may become a useful tool for controlling organic matter build-up and bacterial loads in freshwater RAS.